Section I: Introduction to Biotech laboratory with theory

Section III: Start as Researcher

D. DNA sequencing

F. Thesis writing

Duration and fees: Min 4 to Max 12 months Please enquire for fees by email. Send enquiry to atgcinbiotech@gmail.com

Eligibility: Married women with Master degree from recognized university with gap years before and / or after marriage

Deliverables: 1. Complete confidence in the area of completed project before you enter in the job market; 2. First authorship in DNA and protein database of NCBI; Authorship in research paper whenever communicated for journal, Preparation of CV for job, life time reference for CV, Guidance on phone before interview related to area of guidance

3.  Statement of Purpose (SOP) services 

Areas of interest

1. Molecular Biology: Human Genetics and genomics, Plant Biotech, Animal Biotech, Bacteriology

2. Applied Bioinformatics: Bacterial identification, Male and female gender determination, Barcoding 

3. Hemoglobinopathies: Sickle cell anemia, Alpha and Beta thalassemia, Compound heterozygotes 

4. Virology: Molecular Biology of virus, Viral receptors, Vaccine preparation, Viral detection 

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Invited Guest Lectures from ATG for Educational Institute in and around Pune 

1.       Molecular identification by 16S rRNA gene: Identification of a new bacteria

2.      Molecular identification by ITR: evidence to support 16S rRNA data for identification

3.      Molecular identification by rpoB gene: Identification of very closely related species

4.      DNA barcoding ITS2 for Ayruvedic plants.

5.      Gender determination of plants at juvenile stage.

6.      Comparative genomic studies of viruses by Bioinformatics approach

7.      Sickle cell anemia: Prevalence and management of SCD; field studies of our NGO

8.      Sickle cell anemia & Beta thalassemia: diagnostic kit development

Other lectures and theory at ATG

Special Courses at ATG LAB

Virology: Molecular Biology of The Viruses

Genetics: Human Molecular Genetics and Genomics

Special lectures at ATG LAB

1. Nucleic acid isolation : Human genomic, Bacterial, Paraitic, Mosquito, Plant and Viral DNA & RNA (1hour)

FREE DOWNLOAD

2. Basic PCR lecture series: Basic concepts of PCR and its application in diagnostics and research (1hour)

3. Calculation and setting up of PCR reaction: PCR reaction for conserved region primers (1 hour)

4. PCR Applications : Viral diagnostics, RT PCR and Nested PCR and its variations (1hour)

5. PCR Applications : STRs and Parental detection (1 hour)

6. SNP studies: PCR and DNA sequencing and multiplex PCR, e.g. sickle cell anemia and beta thalassemia (45 min)

7. Post PCR aspects and DNA sequencing: DNA sequence data interpretation (2 hours and 30 min)

8. Applied Bioinformatics 1: NCBI database, selection of interest of gene, BLAST and BLAST 2 (2 hours)

9. Applied Bioinformatics 2 : Phylogenetic analysis (3 hours)

10. Peptide designing in wet lab: Human parvovirus B19 NS1 and VP1 region peptides (2 hours)

11. PCR variation : Based on thermalcycler e.g. Gradient and Touch down PCR (1 hour)

12. PCR variation : Based on template, primers and other PCR reagents (1 hour)

13. PCR standardization and optimization (1 hour)

14. PCR troubleshooting (3 hours)

15. Protein purification : Hemoglobin fraction purification by Ion exchange chromatography(1 hour)

16. Cell and tissue culture : Basic concepts(1 hour)

17. Cell and tissue culture : Applications in viral diagnostics and therapeutics (1 hour)

18. Human Genetic disorders: Thalassemia and sickle cell anemia in India (1 hour)

19. Recombinant DNA technology: Basic concepts (PCR, Ligation, RD, Transformation, PAGE, WB) (1 hour)

20. Recombinant DNA technology: Application to viral vaccine R&D (2 hours)